Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6272036 | Neuroscience | 2015 | 33 Pages |
Abstract
Survivin, a unique member of the inhibitor of the apoptosis protein (IAP) family, has been suggested to play a crucial role in promoting the cell cycle and mediates mitosis during embryonic development. However, the role of survivin following traumatic brain injury (TBI) in adult neurogenesis and apoptosis in the mouse dentate gyrus (DG) remains only partially understood. We adopted adenovirus-mediated RNA interference (RNAi) as a means of suppressing the expression of survivin and observed its effects on adult regeneration and neurological function in mice after brain injury. The mice were subjected to TBI, and the ipsilateral hippocampus was then examined using reverse transcription polymerase chain reaction (RT-PCR) and Western blotting analyses. Brain slices were stained for 5â²-bromo-2â²-deoxyuridine (BrdU) and doublecortin (DCX). Our data showed that survivin knockdown inhibits the proliferation and differentiation of neural precursor cells (NPCs) in the DG of the hippocampus soon after TBI. Furthermore, downregulation of survivin results in a significant increase in programmed cell death in the DG, as assessed using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and 4â²,6-diamidino-2-phenylindole (DAPI) double staining. The Morris water maze (MWM) test was adopted to evaluate neurological function, which confirmed that knockdown of survivin worsened the memory capacity that was already compromised following TBI. Survivin in adult mice brains after TBI can be successfully down-regulated by RNAi, which inhibited adult hippocampal neurogenesis, promoted apoptotic cell death, and resulted in a negative role in the recovery of dysfunction following injury.
Keywords
PCNAPBSshRNAsNSCSGZDAPIPFAIAPDcxMWMLFP4′,6-diamidino-2-phenylindole5′-bromo-2′-deoxyuridineNPCsProliferating Cell Nuclear AntigenBrdUanalysis of varianceANOVAdoublecortinneural stem/progenitor cellNeural precursor cellsdentate gyrusescape latencyMorris water mazePhosphate-buffered salinesubgranular zoneinhibitor of apoptosis proteinparaformaldehydeLateral fluid percussion
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Authors
Z. Zhang, H. Wang, Z. Jin, X. Cai, N. Gao, X. Cui, P. Liu, J. Zhang, S. Yang, X. Yang,