Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6272038 | Neuroscience | 2015 | 10 Pages |
Abstract
Proliferation and differentiation of retinal progenitor cells (RPCs) are tightly controlled by extrinsic cues and distinct combinations of transcription factors leading to the generation of retinal cell type diversity. In this context, we investigated the role of the protein tyrosine phosphatase interacting protein 51 (PTPIP51) in the differentiation of RPCs. The expression pattern of PTPIP51 was analyzed by immunostaining during post-natal retinal development in the rat. Ex vivo electroporation has been used to silence or misexpress PTPIP51 in post-natal retinal explants, and the retinal phenotype was investigated after 3-7Â days in vitro (div). PTPIP51 expression in the retina started postnatally and was maintained throughout adulthood, especially in retinal ganglion cells and in the inner segment of photoreceptor cells. Silencing of Ptpip51 expression in postnatal retina failed to modify the commitment of late RPCs in the different lineages but severely impaired the final differentiation of photoreceptors, observed by a decrease in the fraction of Rhodopsin-positive cells after 7Â div. By contrast, misexpression of PTPIP51 in early or late RPCs failed to modify the differentiation of the RPCs. Our data demonstrate that PTPIP51 is implicated in the differentiation process of immature photoreceptors. Because PTPIP51 is specifically localized in the inner segment, PTPIP51 may contribute to the complex stage of maturation of the apical segment of these cells.
Keywords
ISHNGSMGCsPTPIP51eGFPACSGCLshRNAINLPFARPCsHCsRGCsONLIn situ hybridizationsmall hairpin RNAinner segmentDifferentiationRoom temperatureDIVdays in vitroBCsnormal goat serumAmacrine cellshorizontal cellsRetinal progenitor cellsRetinal ganglion cellsMüller glial cellsBipolar cellsRetinaPhotoreceptorsouter nuclear layerinner nuclear layerganglion cell layerparaformaldehydeenhanced green fluorescent proteinGlutamine synthetase
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Authors
G. Orieux, A. Slembrouck, M. Bensaïd, J.-A. Sahel, O. Goureau,