Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6272508 | Neuroscience | 2015 | 17 Pages |
Abstract
Exposure to an altered osmotic environment during a pre/postnatal period can differentially program the fluid intake and excretion pattern profile in a way that persists until adulthood. However, knowledge about the programing effects on the underlying brain neurochemical circuits of thirst and hydroelectrolyte balance, and its relation with behavioral outputs, is limited. We evaluated whether early voluntary intake of hypertonic NaCl solution may program adult offspring fluid balance, plasma vasopressin, neural activity, and brain vasopressin and angiotensinergic receptor type 1a (AT1a)-receptor gene expression. The manipulation (M) period covered dams from 1Â week before conception until offspring turned 1-month-old. The experimental groups were (i) Free access to hypertonic NaCl solution (0.45Â M NaCl), food (0.18% NaCl) and water [M-Na]; and (ii) Free access to food and water only [M-Ctrol]. Male offspring (2-month-old) were subjected to iv infusion (0.15Â ml/min) of hypertonic (1.5Â M NaCl), isotonic (0.15Â M NaCl) or sham infusion during 20Â min. Cumulative water intake (140Â min) and drinking latency to the first lick were recorded from the start of the infusion. Our results indicate that, after systemic sodium overload, the M-Na group had increased water intake, and diminished neuronal activity (Fos-immunoreactivity) in the subfornical organ (SFO) and nucleus of the solitary tract. They also showed reduced relative vasopressin (AVP)-mRNA and AT1a-mRNA expression at the supraoptic nucleus and SFO, respectively. The data indicate that the availability of a rich source of sodium during the pre/postnatal period induces a long-term effect on drinking, neural activity, and brain gene expression implicated in the control of hydroelectrolyte balance.
Keywords
diaminobenzidine hydrochlorideNHSCVOsBNSTLPBNABPAVPOVLTNTSMNPODRNc-fosPaVSFO5-HTAT1ADABCeAorganum vasculosum of the lamina terminaliscircumventricular organsoxytocinISOphosphate bufferanalyses of varianceANOVALamina terminalisManipulationpostnatal daynormal horse serumSerotoninarea postremaSubfornical organSONArterial blood pressurePVNPalmSupraoptic nucleusbed nucleus of the stria terminalisnucleus of the solitary tractdorsal raphe nucleuslateral parabrachial nucleusparaventricular nucleusmedian preoptic nucleusvasopressinbody weightAntibody
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Authors
A.F. Macchione, C. Beas, F.M. Dadam, X.E. Caeiro, A. Godino, L.F. Ponce, J.L. Amigone, L. Vivas,