Immunolocalization of the P2X4 receptor on neurons and glia in the mammalian retina

Article ID	Journal	Published Year	Pages	File Type
6273551	Neuroscience	2014	17 Pages	PDF

Abstract

By using immunohistochemistry combined with confocal and electron microscopy, the authors demonstrate P2X4 purinergic receptor expression on horizontal, amacrine and ganglion cells, and on macroglia and microglia in the mouse retina. This suggests a role for P2X4-R in the modulation of neuronal and glial signaling and tissue homeostasis in the retina.98

Keywords

IPL protein kinase C-α PKC-α OPL purinergic VGLUT1 INL DAB GCL RT-PCR HEPES GFAP NGS GFP 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid 3,3′-diaminobenzidine Extracellular ATP BSA bovine serum albumin phosphate buffer vesicular glutamate transporter 1 normal goat serum Müller cell outer plexiform layer inner plexiform layer inner nuclear layer ganglion cell layer Microglia Retinal neurons Synapse reverse transcriptase polymerase chain reaction green fluorescent protein Glutamine synthetase P2X4 receptor