Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6274404 | Neuroscience | 2013 | 11 Pages |
Abstract
Microglia have been implicated in disease progression for several age-related brain disorders. However, while microglia's contribution to the progression of these disorders is accepted, the effect of aging on their endogenous cellular characteristics has received limited attention. In fact, a comprehensive study of how the structure and function of microglia changes as a function of developmental age has yet to be performed. Here, we describe the functional response characteristics of primary microglial cultures prepared from embryonic, neonatal (Neo), 2-3 month-old, 6-8 month-old, 9-11 month-old, and 13-15 month-old rats. Microglial morphology, glutamate (GLU) uptake, and release of trophic and inflammatory factors were assessed under basal conditions and in microglia activated with adenosine 5â²-triphosphate (ATP) or lipopolysaccharide. We found that microglia from different age groups were both morphologically and functionally distinct. Upon activation by ATP, Neo microglia were the most reactive, upregulating nitric oxide, tumor necrosis factor-α, and brain-derived neurotrophic factor release as well as GLU uptake. This upregulation translated into neurotoxicity in microglia-neuron co-cultures that were not observed with microglia of different developmental ages. Interestingly, 13-15 month-old microglia exhibited similar activation profiles to Neo microglia, whereas microglia from younger adults and embryos were activated less by ATP. Our data also identify age-dependent differences in purinergic receptor subtype expression that contribute to the regulation of neuronal survival. Combined, our data demonstrate that microglial activation and purinergic receptor profiles vary non-linearly with developmental age, a potentially important finding for studies examining the role of microglia in neurodegenerative disorders.
Keywords
DMEMGFAPIBA1HYPMAP-2FBSLPSPBSBrilliant Blue GBBGNEORB2iNOSBDNFDulbecco’s modified Eagle mediuminducible NO synthasereactive blue 2adenosine 5′-triphosphateATPAminoguanidineenzyme-linked immunosorbent assaysEDTAEthylenediaminetetraacetic acidinflammationanalysis of varianceANOVAELISAtumor necrosis factor-αEmbryonicDIVconditioned mediaDevelopmentdays in vitroAgeingfetal bovine serumNeurotoxicityCytokinesBrain-derived neurotrophic factorTNF-αlipopolysaccharidePhosphate-buffered salineneonatalNitric oxideHypoxiaGlial fibrillary acidic proteinmicrotubule-associated protein-2GluglutamatePurinergic receptors
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Authors
A.Y. Lai, C.D. Dibal, G.A. Armitage, I.R. Winship, K.G. Todd,