Fndc5 knockdown significantly decreased neural differentiation rate of mouse embryonic stem cells

Article ID	Journal	Published Year	Pages	File Type
6275106	Neuroscience	2013	9 Pages	PDF

Abstract

âº Fndc5 knockdown was performed in two stages. âº Fndc5 knockdown during both stages affected neuronal and astrocytes maturation âº Fndc5 expression is required for the appropriate neural differentiation.

Keywords

MEF TRI DAPI HRP PEP NeuN GAPDH MAP2 shRNA EBs TTS GFAP FNDC5 DOX DMEM mESCs LIF PBS BSA NPs Small interfering RNA RNAi short hairpin RNA siRNA bovine serum albumin SKI embryoid bodies standard error of mean doxycycline Retinoic acid Mouse embryonic stem cells cytomegalovirus CMV leukemia inhibitory factor Phosphate-buffered saline Dulbecco’s modified eagle’s medium SEM Neurogenesis neuronal nuclei Horseradish peroxidase Glial fibrillary acidic protein microtubule-associated protein 2 neural progenitors glyceraldehyde 3-phosphate dehydrogenase