Valproic acid induces the glutamate transporter excitatory amino acid transporter-3 in human oligodendroglioma cells

Article ID	Journal	Published Year	Pages	File Type
6275297	Neuroscience	2012	11 Pages	PDF

Abstract

âº Human oligodendroglioma Hs683 cells have no EAAT-dependent amino acid transport. âº Valproic acid (VPA, 1Â mM) induces high-affinity aspartate transport in Hs683 cells. âº Transport stimulation is due to increased SLC1A1 mRNA and EAAT3 protein. âº VPA treatment also induces the early pre-oligodendrocytic marker PDGFRA. âº Trichostatin A mimics VPA effects, pointing to the epigenetic regulation of SLC1A1.

Keywords

TSA PBS PKC TBOA EAAC1 HDAC EBSS PDGF U0126 DTT DHK HEPES PD98059 PDBu 2′-amino-3′-methoxyflavone FBS SDS DMEM 1,4-dithiothreitol 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid DMSO MAPK OPCs Trichostatin A excitatory amino acid carrier 1 dihydrokainate Dimethyl sulfoxide Rarβ Earle’s Balanced Salt Solution sodium dodecyl sulfate fetal bovine serum Oligodendrocyte precursor cells platelet-derived growth factor phorbol 12,13-dibutyrate Phosphate-buffered saline Dulbecco’s modified eagle’s medium histone deacetylases Protein kinase C mitogen-activated protein kinase