| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6275521 | Neuroscience | 2012 | 11 Pages |
Abstract
⺠VAChT genomic DNA is replicated in commercial B6.eGFPChAT mice. ⺠VAChT mRNA expression was elevated in B6.eGFPChAT septal tissue versus B6 controls. ⺠VAChT protein was enhanced in the hippocampus and striatum of B6.eGFPChAT mice. ⺠B6.eGFPChAT mice exhibit enhanced hippocampal acetylcholine release. ⺠Increasing VAChT expression is sufficient to elevate acetylcholine release.
Keywords
Corpus callosumhigh affinity choline transporterThalamic nucleusCHT1BCAqRT-PCRRT-PCRGAPDHTCATBSTrelative expression software tooleGFPmRNACPUqPCRCTXgDNAVAChTPBSDAB3,3-diaminobenzidinecDNATris-buffered saline and Tween 20AChAChEAcetylcholineAcetylcholinesterasetrichloroacetic acidcomplementary deoxyribonucleic acidRNAribonucleic acidhomogenization bufferReSTlateral ventriclebicinchoninic acidvesicular acetylcholine transportermessenger ribonucleic acidSeptummedial septumPhosphate buffered salineCortexknockoutcrossing pointreverse transcriptase polymerase chain reactionQuantitative reverse transcriptase polymerase chain reactionpolymerase chain reactionquantitative polymerase chain reactionPCRCaudate putamenenhanced green fluorescent proteinChATcholine acetyltransferaseCholinergicglyceraldehyde 3-phosphate dehydrogenase
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Authors
P.M. Nagy, I. Aubert,