Crosstalk between cdk5 and MEK-ERK signalling upon opioid receptor stimulation leads to upregulation of activator p25 and MEK1 inhibition in rat brain

Article ID	Journal	Published Year	Pages	File Type
6275663	Neuroscience	2012	14 Pages	PDF

Abstract

âº Acute/chronic opioid receptor stimulation upregulates cdk5 activator p25 in rat brain. âº p25 upregulation is dependent on MEK-ERK and calpain activity. âº Opiate agonists induce p-Thr75 DARPP-32, resulting in PKA inhibition. âº Opiate agonists induce p-Thr286 MEK1, resulting in MEK-ERK inhibition. âº cdk5/ERK crosstalk is important in opiate-induced neuroplasticity.

Keywords

cdk5 MAPK phosphatase-1 ERK kinase MDL28170 MKP-1 SL327 s.c.PARP CTX corpus striatum PP-1 N-methyl-d-aspartate NMDA DARPP-32 ECL Jnk pKa STR PAC ERK BCA i.p.cyclic AMP cAMP DMSO MAPK SDS–PAGE enhanced chemiluminescence bicinchoninic acid Spontaneous withdrawal standard error of the mean intraperitoneal dimethyl sulphoxide subcutaneous cyclin-dependent kinase 5 phosphorylated cerebral cortex MEK SEM protein phosphatase-1 protein kinase A mitogen-activated protein kinase extracellular signal-regulated kinase Opioid receptors