RU486 (mifepristone) ameliorates cognitive dysfunction and reverses the down-regulation of astrocytic N-myc downstream-regulated gene 2 in streptozotocin-induced type-1 diabetic rats

Article ID	Journal	Published Year	Pages	File Type
6276274	Neuroscience	2011	10 Pages	PDF

Abstract

â¶STZ-induced type-1 diabetic rats develop remarkable cognitive impairments 3 mon after diabetes onset. â¶STZ-induced type-1 diabetic rats show significant down-regulation of GFAP and NDRG2 in hippocampus 3 mon after diabetes onset. â¶Nine-day administration with RU486 ameliorates diabetic cognitive dysfunction. â¶Nine-day administration with RU486 reverses the expression of hippocampal GFAP and NDRG2 almost simultaneously.

Keywords

LSD HRP TBS ROD NGS GFAP NGF IOD RIA STZ TBST FBS PBS DAB NDRG2 DCD N-myc downstream-regulated gene 2 RU486 ABC Radio immunoassay cycle threshold streptozotocin agarose gel electrophoresis sodium dodecyl sulfate-polyacrylamide gel electrophoresis SDS-PAGE phosphate buffer analysis of variance ANOVA relative optical density optical densities integrated optical density Tris-buffered saline least significant difference Room temperature fetal bovine serum normal goat serum Age nerve growth factor avidin-biotin-peroxidase complex Phosphate-buffered saline body weight Horseradish peroxidase Glial fibrillary acidic protein Mineralocorticoid receptor glucocorticoids receptor