Lipopolysaccharide-stimulated interleukin-10 release from neonatal spinal cord microglia is potentiated by glutamate

Article ID	Journal	Published Year	Pages	File Type
6276570	Neuroscience	2011	11 Pages	PDF

Abstract

â¶Glutamate significantly increased LPS-stimulated IL-10 release from microglia. â¶The EC50 of glutamate for this potentiation was 103 Î¼M. â¶Glutamate enhanced LPS-stimulated IL-10 release by upregulation of IL-10 mRNA levels. â¶All subtypes of glutamate receptor were involved in the potentiation.

Keywords

PLC tACPD N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl ester)CPCCOEt l-AP4 H-89 U73343 U73122 TLR4 MPEP mGluR PKC MK-801 N-methyl-d-aspartate NMDA pKa LPS RT-PCR APDC IL-10 DHPG 6-Cyano-7-nitroquinoxaline-2,3-dione l-(+)-2-amino-4-phosphonobutyric acid Rp-cAMPS inflammation Interleukin-10 BAPTA-AM ELISA Enzyme-linked immunosorbent assay CNQX phospholipase C EGLU lactate dehydrogenase LDH lipopolysaccharide Microglia Reverse transcriptase-polymerase chain reaction protein kinase A Protein kinase C chelerythrine chloride Kainate Glu glutamate Toll-like receptor 4 Metabotropic glutamate receptor