Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6277085 | Neuroscience | 2010 | 13 Pages |
Abstract
Two monoclonal antibodies (Nilo1 and Nilo2) were generated after immunization of hamsters with E13.5 olfactory bulb-derived mouse neurospheres. They are highly specific for neural stem and early progenitor cell surface antigens. Nilo positive cells present in the adult mouse subventricular zone (SVZ) were able to initiate primary neural stem cell cultures. Moreover, these antibodies added to neurosphere cultures induced proliferation arrest and interfered with their differentiation. In the lateral ventricles of adult mice, Nilo1 stained a cell subpopulation lining the ventricle and cells located in the SVZ, whereas Nilo2 stained a small population associated with the anterior horn of the SVZ at the beginning of the rostral migratory stream. Co-staining of Nilo1 or Nilo2 and neural markers demonstrated that Nilo1 identifies an early neural precursor subpopulation, whereas Nilo2 detects more differentiated neural progenitors. Thus, these antibodies identify distinct neurogenic populations within the SVZ of the lateral ventricle.
Keywords
PSA-NCAMRMSHRPbFGFGFAPECLDcxFITCFCSmAbSGZFACSPBSSVZDABEGFEmbryonic neural stem cellsEGFR3,3′-diaminobenzidineDMEM/F12Monoclonal antibodyimmunoglobulin phosphate bufferRoom temperaturedoublecortinfetal calf serumadult neural stem cellsdentate gyrusepidermal growth factorphycoerythrinphosphate buffer salinefluorescein isothiocyanatefluorescence activated cell sorterElectrochemical luminescencesubgranular zonesubventricular zoneNeurospheresparaformaldehydeHorseradish peroxidaseGlial fibrillary acidic proteinpolyethylene glycolPEGolfactory bulbEpidermal growth factor receptor
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Authors
I. Del Valle, G. Elvira, L. Garcia-Benzaquen, A. Armesilla-Diaz, L. Kremer, J.A. Garcia-Sanz, S. Martinez, A. Silva,