Antinociceptive effect of intrathecal amiloride on neuropathic pain in rats

The aim of this study was to investigate the antinociception of intrathecal amiloride and examine its effect on the neuropathic pain-induced activation of c-Fos and p-p38 MAPK in the rat spinal dorsal horn (SDH). Sprague-Dawley rats were chronically implanted with intrathecal catheters, and the ability of intrathecal amiloride to alleviate nociceptive behaviours in rats with neuropathic pain was tested. Immunohistochemical techniques were utilized to detect the expression of c-Fos and p-p38 in SDH in the control and amiloride (100 μg) groups. Intrathecal amiloride produced dose- and time-dependent antinociception in rats. Additionally, immunohistochemical experiments showed that the expression of c-Fos and p-p38 dramatically decreased in the superficial laminae of the ipsilateral SDH in the 100-μg amiloride group (P < 0.01), whereas, there was no statistical significance on the contralateral side, compared with the control group. Intrathecally administered amiloride develops dose- and time-dependent antinociceptive action in rats with neuropathic pain. It most likely reduces spinal neurons and microglia activation via inhibiting c-Fos and p-p38 MAPK in the SDH of rats.