Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6280946 | Neuroscience Letters | 2015 | 5 Pages |
Abstract
1,8-Cineole is a terpenoid present in many essential oil of plants with several pharmacological and biological effects, including antinociceptive, smooth muscle relaxant and ion channel activation. Also, 1,8-cineole blocked action potentials, reducing excitability of peripheral neurons. The objective of this work was to investigate effects of 1,8-cineole on Na+ currents (INa+) in dissociated superior cervical ganglion neurons (SCG). Wistar rats of both sexes were used (10-12 weeks old, 200-300Â g). SCG's were dissected and neurons were enzymatically treated. To study 1,8-cineole effect on INa+, the patch-clamp technique in whole-cell mode was employed. 1,8-Cineole (6.0Â mM) partially blocked INa+ in SCG neurons. The effect stabilized within â¼150Â s and there was a partial recovery of INa+ after washout. Current density was reduced from â105.8 to â83.7Â pA/pF, corresponding to a decrease to â¼20% of control. 1,8-Cineole also reduced the time-to-peak of INa+ activation and the amplitude and decay time constants of INa+ inactivation. Current-voltage plots revealed that 1,8-cineole left-shifted the V1/2 of both activation and inactivation curves by â¼10 and â¼20Â mV, respectively. In conclusion, we demonstrate that 1,8-cineole directly affects Na+ channels of the SCG by modifying several gating parameters that are likely to be the major cause of excitability blockade.
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Authors
Francisco Walber Ferreira-da-Silva, Kerly Shamyra da Silva-Alves, ThaÃs Antônia Alves-Fernandes, Andrelina Noronha Coelho-de-Souza, José Henrique Leal-Cardoso,