Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6282294 | Neuroscience Letters | 2014 | 5 Pages |
â¢We found that Gap43 and Ntn1 were up-regulated by IGF-1 in the process of hair cell protection.â¢The expression of Gap43 and Ntn1 was dependent on both the PI3K/Akt and MEK/ERK pathways.â¢The up-regulation of Gap43 and Ntn1 transcripts were transient.
We previously reported that insulin-like growth factor 1 (IGF-1) protects cochlear hair cells against aminoglycosides through activation of the PI3K/Akt and MEK/ERK pathways in supporting cells. In this study, we found that IGF-1 up-regulated the expression levels of Gap43 and Ntn1 as measured using cDNA microarray analysis and qRT-PCR. Using inhibitors of the PI3K/Akt and MEK/ERK pathways, we reveal that both pathways are involved in the up-regulation of Gap43 and Ntn1 expression. Moreover the time window of Gap43 and Ntn1 transcription was limited to within 12Â h after IGF-1 treatment, indicating that downstream gene expression was tightly controlled by IGF-1.