Three-dimensional ultra-structures of myelin and the axons in the spinal cord: Application of SEM with the osmium maceration method to the central nervous system in two mouse models

► SEM with osmium maceration method can observe three-dimensional structures of myelin and axons. ► This technique left myelin and axonal structures undisturbed. ► This technique can observe complicated abnormalities of myelin and axons in the EAE spinal cord.