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RNA analysis of inner ear cells from formalin fixed paraffin embedded (FFPE) archival human temporal bone section using laser microdissection - A technical report

Article ID Journal Published Year Pages File Type
6287535 Hearing Research 2013 6 Pages PDF
Abstract
We detected COCH and SLC26A5 mRNA in specific structures and cells of the inner ear from archival human temporal bone. Our innovative method using laser microdissection and semi-nested RT-PCR should advance future RNA study of human inner ear diseases.
Keywords
OHCLMDSGCMitochondrial DNAFFPElaser microdissectionEDTAEthylenediaminetetraacetic acidmtDNAspiral ligamentOuter hair cellsspiral ganglion cellsStria vascularisformalin fixed paraffin embedded
Related Topics
Life Sciences Neuroscience Sensory Systems
Preview
RNA analysis of inner ear cells from formalin fixed paraffin embedded (FFPE) archival human temporal bone section using laser microdissection - A technical report
Authors
Yurika Kimura, Sachiho Kubo, Hiroko Koda, Kazuhiro Shigemoto, Motoji Sawabe, Ken Kitamura,
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Journal
Hearing Research
Journal: Hearing Research
Related Categories
OHC
LMD
SGC
Mitochondrial DNA
FFPE
laser microdissection
EDTA
Ethylenediaminetetraacetic acid
mtDNA
spiral ligament
Outer hair cells
spiral ganglion cells
Stria vascularis
formalin fixed paraffin embedded
Behavioral Neuroscience
Biological Psychiatry
Cellular and Molecular Neuroscience
Cognitive Neuroscience
Developmental Neuroscience
Endocrine and Autonomic Systems
Neurology
Neuroscience (General)
Sensory Systems
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