| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6288202 | Research in Microbiology | 2008 | 8 Pages |
Abstract
The integrase (Int) protein of coliphage HK022 can catalyze in Escherichia coli as well as in in vitro integrative and excisive recombinase-mediated cassette exchange reactions between plasmids as substrates. Atomic force microscopy images have revealed that in the protein-DNA complexes that are formed, the plasmid substrates are connected via one and not two pairs of attachment sites. This observation, together with the elucidation of intermediate co-integrates between the two circular plasmids, suggest that a sequential mechanism of the RMCE reaction is possible.
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Authors
Natalia Malchin, Tatiana Molotsky, Ezra Yagil, Alexander B. Kotlyar, Mikhail Kolot,