Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6290899 | Experimental Parasitology | 2014 | 5 Pages |
Abstract
Previously generated two auxotrophic genes knockout Edwardsiella tarda (Îalr Îasd E. tarda) was used as a delivery vehicle for CpG 1668 motif-enriched plasmids (pL-CpG30), and potential of the Îalr Îasd E. tarda-mediated delivery of CpG motifs in both prevention and therapeutic treatment of scuticociliatosis caused by Miamiensis avidus in olive flounder (Paralichthys olivaceus) was investigated. The auxotrophic E. tarda mutant with pL-CpG30 plasmids elicited evidently higher survival rates and delayed both onset of mortality and time-to-death of olive flounder against M. avidus challenge. Furthermore, administration of E. tarda mutant that contains plasmids enriched in CpG 1668 motif elicited clearly higher survival rates of fish that were already infected with M. avidus. These results indicate that CpG 1668 plasmids-harboring E. tarda mutant may provide not only preventive measure but also therapeutic approach against scuticociliatosis in olive flounder.
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Parasitology
Authors
Yue Jai Kang, Seung Hyuk Choi, Ki Hong Kim,