| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6291568 | Experimental Parasitology | 2012 | 5 Pages |
Abstract
⺠The macrophage-amastigote model is used for testing drugs against Leishmania species. ⺠The activity of the test drug is measured by microscopic counting. ⺠Microscopic counting is a time-consuming and subjective method of analysis. ⺠qPCR assay replaces readouts of number of amastigote per macrophages by DNA load measurement. ⺠qPCR assay increase the throughput of drug screening using intracellular amastigotes assays.
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Authors
Luciana I. Gomes, Felipe M. Gonzaga, Eliane de Morais-Teixeira, Bruna S. de Souza-Lima, Verônica V. Freire, Ana Rabello,