Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6291661 | Experimental Parasitology | 2011 | 6 Pages |
Abstract
⺠Effects of nonesterified fatty acids (NEFA) and phospholipids on intraerythrocytic growth of Plasmodium falciparum were investigated in serum-free medium. ⺠Different combinations of NEFA had variable effects on each developmental stage of P. falciparum in the presence or absence of phospholipids, depending on certain structural characteristics of NEFA, such as carbon-chain length, degree and position of unsaturation, and isomerism. ⺠Four typical growth patterns indicating complete growth, suppressed schizogony, suppressed formation of merozoites, and inhibited invasion of merozoites resulted from culture in various combinations of lipids. ⺠Different NEFA exerted distinct roles in erythrocytic growth of the parasite by sustaining development at different stages.
Keywords
GFScis-9-octadecenoic acidcRPMIC14:0C12:0Tetradecanoic acidC16:1C18:0BSAFRFUPLDNEFAIMIDocosanoic acidRBCPRBCFCMSMFC16:0C18:2ErythrocytesOctadecanoic acidDodecanoic acidHexadecanoic acidNon-esterified fatty acidBasal mediumHuman serumphosphatidylcholinePhospholipidsFlow cytometrySerum-free mediumrelative fluorescent unitsPlasmodium falciparum
Related Topics
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Immunology and Microbiology
Parasitology
Authors
Hiroko Asahi, Shinji Izumiyama, Mohammed Essa Marghany Tolba, Bethel Kwansa-Bentum,