Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6291882 | Experimental Parasitology | 2011 | 5 Pages |
Chemical acaricides, especially fipronil (active ingredient of Frontline®), are still the most effective method to control tick populations. In this study, the effectiveness of fipronil was assessed in semi-engorged females of the tick Rhipicephalus sanguineus. A protocol for an in vitro bioassay (AIT) was developed, and the LC50 (lethal concentration 50%) and 95% confidence interval were determined. Ticks were immersed in Petri dishes with different concentrations of fipronil or distilled water for 2Â min, dried, and placed in an incubator for 7Â days. Dead R. sanguineus females treated with the 14 concentrations of fipronil were counted daily. Mortality results were compared with the Probit analysis, and the LC50 and 95% confidence interval were calculated, g (95): LC50Â =Â 9.647 (4.711 to 13.470). This study was aimed at developing a more appropriate and updated protocol for an in vitro bioassay (AIT - adult immersion test), and providing information on the toxic potential of fipronil (elimination of ectoparasites with lower concentrations) and sensitivity of ticks, especially R. sanguineus, a pest of great interest, due to its occurrence in urban environments.
Graphical abstractThe present study was aimed at developing a more appropriate and updated protocol for an in vitro bioassay (AIT - adult immersion test), and providing information on the toxic potential of fipronil (elimination of ectoparasites with lower concentrations) and sensitivity of ticks, especially R. sanguineus. The LC50 and the 95% confidence interval were also determined.Download full-size imageResearch highlights⺠A more appropriate and updated protocol for an in vitro bioassay (AIT - adult immersion test). ⺠Providing information on the toxic potential of fipronil (elimination of ectoparasites with lower concentrations). ⺠Sensitivity of ticks, especially R. sanguineus, a pest of great interest, due to its occurrence in urban environments.