| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6291940 | Experimental Parasitology | 2011 | 4 Pages |
Abstract
⺠This study aim to develop a simple and reliable method to determine the viability of Giardia intestinalis after incubation with an anti-giardial agent by using a colorimetric method. ⺠Giardia intestinalis trophozoites are cultured in 96-well plate, fixed, stained with 0.1% methylene blue and the intensity of dye is determined by microplate reader. ⺠OD values from the unwashed wells before fixing with methanol were significantly higher than that from the washed wells. ⺠A better result was obtained when the fixed trophozoites were carefully washed by immersing the whole plate into the washing solution at each step. ⺠The IC50 and IC90 of metronidazole, ornidazole and furazolidone as calculated from our method and the conventional method are comparable.
Related Topics
Life Sciences
Immunology and Microbiology
Parasitology
Authors
Kruawan Hounkong, Nongyao Sawangjaroen, Souwalak Phongpaichit,