Research BriefMolecular identification of three Trichinella isolates from Heilongjiang Province, People's Republic of China

DNAs of Trichinella dog isolate (HC), Trichinella swine isolate (HH) and Trichinella cat isolate (SW), obtained from Heilongjiang Province, were amplified by the fragments of 18S rDNA and ITS2. Two reference strains, Trichinella spiralis (ISS3) and Trichinella nativa (ISS10) were used for sequence comparison. Sequence and dendrogram analysis indicated that HC belonged to T. nativa and HH together with SW belonged to T. spiralis. This method permits rapid species identification of Trichinella isolates, although further evaluation is required before precisely identification.

► Trichinella dog isolate (HC), Trichinella swine isolate (HH) and Trichinella cat isolate (SW) were amplified by the fragments of 18S rDNA and ITS2. Two reference strains, Trichinella spiralis (ISS3) and Trichinella nativa (ISS10) were used for sequence comparison. In this study, the 18S rDNA and ITS2 genes were cloned and analyzed from Trichinella spiralis (ISS3), Trichinella nativa (ISS10) and three Trichinella isolates from Heilongjiang, China. Sequence and dendrogram analysis indicated that HC belonged to Trichinella nativa and HH together with SW belonged to Trichinella spiralis. The results showed that Trichinella isolates could be identified and classified by this method.