Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6292008 | Experimental Parasitology | 2012 | 7 Pages |
Abstract
⺠Triosephosphate isomerase from Leishmania donovani was cloned and characterized. ⺠Recombinant LdTIM had pH optima in the range of 7.2-9.0 and found stable at 25 °C for 30 min. ⺠The Km and Vmax for glyceraldehyde 3-phosphate were 0.32 mM and 10.05 mM/min/mg, respectively. ⺠Methylmethane thiosulphonate induced 75% enzyme inactivation within 15 min at 250 μM.
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Authors
Kishore Kumar, Prachi Bhargava, Uma Roy,