Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6305194 | Journal of Great Lakes Research | 2015 | 5 Pages |
Abstract
Membrane filtration/culture techniques have been demonstrated to be reliable and broadly applicable for determination of fecal contamination in recreational waters. The time-consuming nature of culture techniques, however, is viewed as the major disadvantage of this type of analysis, and many authors have asserted the need for improved rapid-detection methods. In this study, we evaluated the performance of the ENDETEC⢠TECTA⢠B16, an automated fluorometry-based microbial detection system, by comparing its detection time and accuracy to those of two common culture-based methods, which are widely-used for recreational water quality monitoring in Canada. Our results demonstrated that Escherichia coli densities inferred by the TECTA⢠method were generally in agreement with those generated by standard culture methods (y = 1.19x + 0.002, R2 = 0.89) and under the current calibration regime, TECTA⢠tended to slightly overestimate E. coli densities. In addition, TECTA⢠was able to detect E. coli densities in exceedance of the Ontario Provincial Water Quality Objective for recreational waters in as little as 4 h (mean detection time = 7.03 h), representing a substantial improvement over traditional culture techniques. Our findings suggest that with improved calibration, TECTA⢠may provide a viable, cost-effective, rapid alternative to culture approaches for the detection of fecal contamination in recreational waters.
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Earth and Planetary Sciences (General)
Authors
Andrew J. Bramburger, R. Stephen Brown, Jennifer Haley, Jeffrey J. Ridal,