Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6306535 | Chemosphere | 2016 | 6 Pages |
Abstract
Performing environmental assessments for double-stranded RNA-based agricultural products require the development of sensitive and selective methods to measure biodegradation rates of dsRNAs. We developed and characterized a novel analytical procedure that uses a molecular hybridization assay (QuantiGene®) to accurately measure dsRNA extracted from diverse soils. In this report, we utilize this method to demonstrate that two dsRNAs with distinct size, structure, and sequence degrade rapidly in soil with indistinguishable kinetics.
Keywords
Related Topics
Life Sciences
Environmental Science
Environmental Chemistry
Authors
Joshua R. Fischer, Fatima Zapata, Samuel Dubelman, Geoffrey M. Mueller, Peter D. Jensen, Steven L. Levine,