The chemical composition and source identification of soil dissolved organic matter in riparian buffer zones from Chongming Island, China

Dissolved organic matter (DOM) that is derived from the soil of riparian buffer zones has a complex chemical composition, and it plays an important role in the transport and transformation of pollutants. To identify the source of DOM and to better understand its chemical and structural properties, we collected 33 soil samples from zones with fluctuating water levels along the major rivers on Chongming Island, evaluated the DOM contents in riparian soil, analyzed the chemical composition and functional groups and traced DOM origins by using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) combined with clustering analysis. All sampling sites were divided into four groups by principal component analysis (PCA) on the basis of the DOM molecules. The results showed that there was no significant difference in the DOM contents between every two groups; however, the DOM fractions differed significantly among the different site groups in the following order: Σ lipids and Σ proteins > Σ sugars and Σ fatty acids > Σ amino acids, Σ indoles and Σ alkaloids. DOM in the riparian buffer zones originated from riparian plants, domestic sewage and agricultural activities, and the hydrophobic and amphiphilic fractions accounting for over 60% of the identified molecules were the dominant fractions. Our study has confirmed the heterogeneous properties of DOM, and it is of vital importance to isolate and characterize the various DOM fractions at the molecular level for a better understanding of the behavior and roles of DOM in the natural environment.