Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6308901 | Chemosphere | 2014 | 5 Pages |
Abstract
REMI (restriction enzyme-mediated integration) technique was employed to construct Trichoderma atroviride strain T23 mutants with degrading capability of neonicotinoid insecticide, imidacloprid. The plasmid pBluescript II KS-hph used for integration in REMI mutants was confirmed by PCR and Southern hybridization. Among 153 transformants, 57% of them have showed higher neonicotinoid insecticide, imidacloprid, degradation ability than the wild strain T23 (p < 0.01). More specifically, seven single-copied T. atroviride T23 transformants have confirmed a 30% higher degradation rate than their parent isolate. Among all transformed mutants, a 95% imidacloprid degradation rate was identified as the highest. This study, thus, provided an effective approach for improving neonicotinoid insecticide-degrading capability using REMI transformed T. atroviride mutants.
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Authors
Xiangfeng He, Abebe Jenberie Wubie, Qingyun Diao, Wei Li, Fei Xue, Zhanbo Guo, Ting Zhou, Shufa Xu,