Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6310157 | Chemosphere | 2015 | 5 Pages |
Abstract
This present study reports that reactive oxygen species (ROS), which mainly consist of hydroxyl (OH) and superoxide (O2-) radicals generated by the photocatalysis of TiO2 nanoparticles (TiO2 NPs) in suspension, can be quantitatively measured by the colorimetric probe-assisted spectrometric method without any interference by the TiO2 NPs. The interference effect of the TiO2 NPs with UV-vis spectroscopy was excluded by the selective separation of p-nitrosodimethylaniline (RNO) and tetrazolium salt (XTT) (probe compounds for OH and O2-, respectively) from the TiO2 NPs in the suspension by centrifugation at a specified ionic strength adjusted with phosphate buffer (KH2PO4/NaOH, PB) solution. This colorimetric probe-assisted spectrometric method was also successfully applied to measure the steady-state production of photogenerated ROS in a TiO2 NPs suspension.
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Authors
Choonsoo Kim, Hee-jin Park, Seoeun Cha, Jeyong Yoon,