Article ID Journal Published Year Pages File Type
6312261 Ecotoxicology and Environmental Safety 2014 5 Pages PDF
Abstract

•Out of 318 food and feed samples analyzed by DR CALUX bioassay 45 samples (14 percent) exceeded EU maximum limits.•DR CALUX bioassay BEQs measured in food and feed ranged 0.10-5.90.•The dietary intakes of males and females were in the range of the WHO-TDI range of 1-4 WHO-TEQ kg−1 body weight day−1.

A total of 318 local and imported meat, milk, eggs, fish, and animal feed samples collected in Kuwait were analyzed by cell-based reporter gene assay (Dioxin-Responsive Chemical Activated LUciferase gene eXpression DR-CALUX) for PCDD/Fs and dl-PCBs. The bioanalytical equivalents (BEQs) obtained by DR-CALUX bioassay were compared with the official maximum limits according to the European Commission (EC) regulations. Suspected and randomly chosen negative samples were analyzed by gas chromatography-high resolution mass spectrometry (GC-HRMS). The results showed that among suspected samples, one sample was confirmed to be non-compliant. The positive sample was of imported origin. The correlation coefficient of 0.98 between DR-CALUX and GC-HRMS was found. Moreover, the average daily intakes of PCDD/Fs and dl-PCBs for the Kuwaiti population were estimated. Results obtained in this study were discussed and compared with other published data.

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