Plasminogen purification from serum through affinity membranes

In this work, we prepared affinity membranes for plasminogen purification using l-lysine as affinity ligand. To this aim regenerated cellulose membranes were used as a support for ligand immobilization. The efficiency of l-lysine coupling was inspected by applying different binding protocols and the immobilization yield was studied as a function of the reaction conditions. The membranes have been characterized in batch and in complete chromatographic cycles using bovine and human serum as test probes. The results obtained indicate that the l-lysine affinity membranes are a promising alternative for the purification of plasminogen.