Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
634240 | Journal of Membrane Science | 2013 | 10 Pages |
Abstract
Adsorption and desorption of bovine serum albumin (BSA) and immunoglobulin G (IgG) have been investigated. Loading was conducted in high ionic strength buffer. Elution was conducted in low ionic strength buffer. By using a responsive ligand that changes its conformation during loading and elution, high protein recoveries were obtained. Ligands that respond to solution ionic strength show promise for high performance hydrophobic interaction chromatography.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Filtration and Separation
Authors
Heath H. Himstedt, Xianghong Qian, Justin R. Weaver, S. Ranil Wickramasinghe,