Article ID Journal Published Year Pages File Type
6356291 Marine Pollution Bulletin 2016 6 Pages PDF
Abstract

•Oxidative stress markers (EA, TAC, TOS) were proposed to be useful in biomonitoring.•K-phosphate buffer was chosen for EA, TAC, TOS extraction from gills of mussels.•The validated methods were precise and accurate.•EA, TAC, TOS were stable for at least 4 months in prepared samples.•EA, TOS and TAC showed changes with pollution.

The aims of the present study were to optimize and validate methods for esterase activity (EA), total oxidant status (TOS) and total antioxidant capacity (TAC) determination in mussel' gills, and to establish the relationships between these biomarkers and Pb, Cd and Cu pollution, in single form and ternary mixture. Two different buffers for sample homogenization, the need of ultracentrifugation, and analytical validation were evaluated. Coefficients of variation, when buffer without additives and ultracentrifugation were used, were < 15%, and recovery were 97%-109% in all cases. The EA response tends to decrease with treatments, TOS decreased significantly in Cd and ternary groups, while TAC tended to increase in treatments with Pb, Cd and ternary groups. In conclusion, the methods for EA, TOS and TAC measurements in gills of mussel were precise and accurate and could be interesting resources in biomonitoring programmes.

Related Topics
Physical Sciences and Engineering Earth and Planetary Sciences Oceanography
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