Hydrolysis rate constants at 10-25 °C can be more than doubled by a short anaerobic pre-hydrolysis at 35 °C

For low temperature hydrolysis with the pre-hydrolysis at 35 °C, hydrolytic enzymes produced at the start temperature of 35 °C were still active when decreasing the temperature. These relatively high number of enzymes after applying the pre-hydrolysis at 35 °C resulted in high hydrolysis rate constants at low temperatures (10-25 °C) compared with those without the pre-hydrolysis.228