A preparation of a single-layered enzyme-membrane using asymmetric pBPPO base film for development of pesticide detecting biosensor

A novel single-layered enzyme-membrane using BPPO film which was pore-filled with cross-linked PVA containing TYR was prepared to develop a pesticide biosensor with an increased stability. The prepared enzyme-membrane was assembled on a glassy carbon electrode to detect pesticides. The voltammetric measurements revealed a coupled reaction, an enzymatic oxidation and an electrochemical reduction of catechol transported through the prepared membrane. A detection range of parathion and carbaryl was 0.01–1 ppb and 0.01–10 ppb, respectively. The activity in the enzyme-membrane was maintained for 1 month due to favorable aqueous environment of PVA for enzyme activity while BPPO film provided structural stability.