Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6387668 | Marine Environmental Research | 2015 | 10 Pages |
Abstract
Eleven putative individual GSTs from classes Mu, Pi and Sigma were identified by proteomics. Few variations were observed in total GST activity of post-mitochondrial samples between sampling sites, with animals from Matosinhos (polluted site) showing highest GST activity and Cabo Home (clean site) showing lowest. This contrasts with the increased number of differences in the individual GST isoforms. Each mussel population showed unique GST proteomic profiles. Based on the results we conclude that proteomics surpasses the conventional GST enzymatic activity method to discriminate natural mussel populations and has potential application in environmental monitoring. It is reasonable to suggest that the GST proteomic profiles observed may reflect differences in contamination levels.
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Authors
Catarina C. Azevedo, Remédios Guzmán-Guillén, José C. Martins, Hugo Osório, Vitor Vasconcelos, Rute R. da Fonseca, Alexandre Campos,