Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6387861 | Marine Environmental Research | 2013 | 8 Pages |
Abstract
The ability of 17α-ethinylestradiol (EE2) to elevate vitellogenin levels were investigated in male flounder Platichthys flesus and vitellogenin concentrations in flounders from the Danish coastal environment were determined. Male flounders were exposed to 17α-ethinylestradiol (EE2) via food or water. Average vitellogenin concentrations in the control fish ranged between 25 and 100 ng mLâ1. Exposure to 5.1, 8.1 and 16.8 ng EE2 Lâ1 in water and 500 and 5000 ng EE2 kgâ1 body weight (bw) every second day in the food increased the plasma vitellogenin concentration in a concentration and time dependent manner, whereas exposure to 2.7 ng EE2 Lâ1 in water for 21 d and 5 and 50 ng EE2 kgâ1 bw for 12 days in the food did not. EE2 could be detected in liver and testes (but not in muscle) after exposure to 8.1 and 16.8 ng EE2 Lâ1 in the water and 5000 ng EE2 kgâ1 bw in the food; the highest concentration was 6 ng gâ1 wet weight in liver. The majority of the male flounders collected from nine coastal Danish sites from 1999 to 2004 had vitellogenin concentrations below 100 ng mLâ1, and only at two sites moderate estrogenic inputs were indicated.
Keywords
Related Topics
Physical Sciences and Engineering
Earth and Planetary Sciences
Oceanography
Authors
Louise Leonharder Madsen, Bodil Korsgaard, Knud Ladegaard Pedersen, Lisette Bachmann Bjerregaard, Thomas Aagaard, Poul Bjerregaard,