Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6387949 | Marine Environmental Research | 2013 | 10 Pages |
â¢Three new cytochrome P450 (CYP) genes were cloned in the polychaete Perinereis nuntia.â¢P. nuntia CYP431A1 is involved in the detoxification of the water accommodated fraction of crude oil.â¢CYP3 clan gene also plays an important role in B[α]P metabolisms.â¢Phase II and antioxidant enzymes were induced following PAH exposure in P. nuntia.â¢P. nuntia has a potential to be used as indicator for WAF and B[α]P pollution in sediments.
To report a novel CYP genes and to evaluate its potency as a biomarker for oil pollution, we cloned three CYP genes and measured their expression profiles under controlled lab conditions using real-time reverse transcription PCR (real-time RT-PCR) after exposure of the water accommodated fraction (WAF) of Iranian crude oil and benzo[α]pyrene (B[α]P) as a positive control. Of these, CYP432A1 (CYP3 clan) gene was significantly induced by B[α]P exposure, indicating that the CYP3 clan gene would play an important role in polycyclic aromatic hydrocarbon (PAH) metabolisms, particularly for B[α]P in this species. However, the Perinereis nuntia CYP431A1 mRNA, a CYP2 clan gene, was sensitively expressed to WAF exposure with other two CYP genes. As one of Phase II detoxification enzymes, the glutathione S-transferase (GST) genes also upregulated with other antioxidant genes (SOD and CAT), indicating that WAF-exposed P. nuntia was properly responding to this kind of chemical stress. Thus, three CYP genes from the polychaete, P. nuntia have a potential as a biomarker in monitoring of the marine sediment after an oil spill accident.
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