Article ID Journal Published Year Pages File Type
6387949 Marine Environmental Research 2013 10 Pages PDF
Abstract

•Three new cytochrome P450 (CYP) genes were cloned in the polychaete Perinereis nuntia.•P. nuntia CYP431A1 is involved in the detoxification of the water accommodated fraction of crude oil.•CYP3 clan gene also plays an important role in B[α]P metabolisms.•Phase II and antioxidant enzymes were induced following PAH exposure in P. nuntia.•P. nuntia has a potential to be used as indicator for WAF and B[α]P pollution in sediments.

To report a novel CYP genes and to evaluate its potency as a biomarker for oil pollution, we cloned three CYP genes and measured their expression profiles under controlled lab conditions using real-time reverse transcription PCR (real-time RT-PCR) after exposure of the water accommodated fraction (WAF) of Iranian crude oil and benzo[α]pyrene (B[α]P) as a positive control. Of these, CYP432A1 (CYP3 clan) gene was significantly induced by B[α]P exposure, indicating that the CYP3 clan gene would play an important role in polycyclic aromatic hydrocarbon (PAH) metabolisms, particularly for B[α]P in this species. However, the Perinereis nuntia CYP431A1 mRNA, a CYP2 clan gene, was sensitively expressed to WAF exposure with other two CYP genes. As one of Phase II detoxification enzymes, the glutathione S-transferase (GST) genes also upregulated with other antioxidant genes (SOD and CAT), indicating that WAF-exposed P. nuntia was properly responding to this kind of chemical stress. Thus, three CYP genes from the polychaete, P. nuntia have a potential as a biomarker in monitoring of the marine sediment after an oil spill accident.

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Physical Sciences and Engineering Earth and Planetary Sciences Oceanography
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