Article ID Journal Published Year Pages File Type
639448 Journal of Membrane Science 2006 8 Pages PDF
Abstract

A highly instrumented pilot microfiltration (MF) plant featuring a ceramic microfiltration membrane, back-pulsing device, permeate re-circulation in co-flow to achieve uniform transmembrane pressure (UTMP) and a cooling/temperature control system was designed, built and tested successfully with transgenic whole goat milk (TGM). In a previous study, it was shown that >90% of heterologous IgG could be recovered from TGM in the permeate by adopting close process control with low UTMP and operation at the iso-electric point (pI) of the target IgG. Here, these concepts were successfully employed to recover >90% of another IgG (pI = 7.1–7.5) from TGM by MF at the pilot-scale. The pilot plant MF system gave 90% yield for the same target protein with a 50% higher permeation flux rate (47 lmh instead of 32 lmh) and increased protein transmission (70% instead of 46%) as compared with the laboratory-scale MF unit. Thus, this study demonstrated the efficacy of the pilot MF plant and reinforced the generality at larger scale of the optimization methodology reported earlier by us.

Related Topics
Physical Sciences and Engineering Chemical Engineering Filtration and Separation
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