| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 640663 | Separation and Purification Technology | 2015 | 11 Pages |
•MAATPE combined microwave-assisted extraction (MAE) with aqueous two-phase extraction (ATPE).•Extraction and purification were integrated in one step for alkaloids from the herb material.•Screening of aqueous two-phase extraction system (ATPS) and extraction conditions were investigated in details.•MAATPE can achieve the higher yield and recovery of alkaloids than two-step methods.•The mechanism for MAATPE was discussed based on a novel model of multiple-phase extractions.
A rapid and effective method of integrating extraction and purification for alkaloids from Sophora flavescens Ait. was developed by microwave-assisted aqueous two-phase extraction (MAATPE) based on the high efficiency of microwave-assisted extraction (MAE) and the demixing effect of aqueous two-phase extraction (ATPE). The aqueous two-phase system (ATPS), ethanol/ammonia sulfate was chosen from seven combinations of ethanol/salt systems, and its extraction properties were investigated in detail. Key factors, namely, the compositions of ATPS, solvent-to-materials ratio, and the extraction temperature were selected for optimization of the experimental conditions using response surface methodology (RSM) on the basis of the results of the single-factor experiment. The final optimized conditions were, the compositions of ATPS: ethanol 28% (w/w) and (NH4)2SO4 18% (w/w), solvent-to-material ratio 60:1, temperature 90 °C, extraction time 5 min, and microwave power 780 W. MAATPE was superior to MAE, the latter using a single solvent, not only in extraction yield but also in impurity content. Moreover, compared with the combination of MAE and ATPE in the two-step mode, MAATP demonstrated fewer impurities, a better yield (63.78 ± 0.45 mg/g) and a higher recovery (92.09 ± 0.14%) in the extraction and purification of alkaloids. A continuous multiphase-extraction model of MAATPE was proposed to explicate the extraction mechanism. MAATPE revealed that the interaction between microwave and ATPS cannot only cause plant cell rupture but also accelerate demixing, improving mass-transfer from solid–liquid extraction to liquid–liquid purification. MAATPE simplified procedures also contributed to the lower loss occurrence, better extraction efficiency, and reduced impurity to target constituents.
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