Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
642809 | Separation and Purification Technology | 2010 | 4 Pages |
Abstract
High-speed countercurrent chromatography (HSCCC) was applied to preparative separation and purification of punicalin from pomegranate husk by one-step separation. The two-phase system consisted of n-butyl alcohol–ethyl acetate–water (4:1:5, v/v/v) was employed. The revolution speed of the separation column, flow rate of the mobile phase and separation temperature were 800 rpm, 2 ml/min and 25 °C respectively. About 100 mg amount of crude extract was separated, yielding 13.1 mg of punicalin at a high purity of over 96%. The mass recovery was more than 90%. The product was assessed by analytical HPLC and characterized by MS, 1H NMR and 13C NMR.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Filtration and Separation
Authors
Honghao Zhou, Jiao Lv, Qipeng Yuan,