Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
643429 | Separation and Purification Technology | 2008 | 4 Pages |
Abstract
Chlorogenic acid, caffeic acid and luteolin are the major phenolic compounds found in the traditional Chinese herbal medicine Caulis Lonicerae. The separation and purification of the three compounds from the crude extract of Caulis Lonicerae was achieved by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of ethyl acetate–ethanol–water (4:1:5, v/v). Caffeic acid (7.2 mg), chlorogenic acid (15.7 mg) and luteolin (18.8 mg) from 110 mg crude extract of Caulis Lonicerae were obtained and their purities were 95.55%, 97.24% and 98.11%, respectively.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Filtration and Separation
Authors
Zhilong Wang, Jianhua Wang, Yinshi Sun, Shengbo Li, Hanzhong Wang,