Article ID Journal Published Year Pages File Type
643709 Separation and Purification Technology 2008 6 Pages PDF
Abstract

Four acetophenones, baishouwubenzophenone (1), 4-hydroxyacetophenone (2), 2,4-dihydroxyacetophenone (3) and 2,5-dihydroxyacetophenone (4), were successfully isolated and purified from the Chinese medicinal plant Cynanchum bungei Decne. by high-speed counter-current chromatography (HSCCC) using a pair of two solvent system, one consisting of light petroleum–ethyl acetate–methanol–water (2:5:3:3, v/v) and another of light petroleum–ethyl acetate–methanol–water (2:4:3:3, v/v). A total of 62.3 mg (1), 18.9 mg (2), 15.0 mg (3) and 4.5 mg (4) were purified from the n-butanol extract (600 mg) of crude sample of C. bungei Decne. in one-step separation within 5 h, each at over 96% purity as determined by high performance liquid chromatography (HPLC). Their structures were identified by Mass Spectrometer (MS), Nuclear magnetic resonance (1H NMR and 13C NMR).

Related Topics
Physical Sciences and Engineering Chemical Engineering Filtration and Separation
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