| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 643824 | Separation and Purification Technology | 2008 | 4 Pages |
Abstract
An 8.1% polyethyleneglycol (PEG) 300/22.2% phosphate aqueous two-phase system was used to obtain a clear extract of the earthworm Eisenia fetida. Lumbrokinase was then purified from the extract by anion-exchange chromatography at pH 4.6. 80% enzyme activity was recovered with a purification factor of 8.0 and 97% purity. No further purification was achieved by submitting the final product to chromatography on benzamidine-agarose.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Filtration and Separation
Authors
N.B. Iannucci, S.A. Camperi, O. Cascone,