The effect of pulsed electric field on DNA extraction from bovine spleens

We developed a high throughput DNA extraction method from bovine spleen with pulsed electric fields (PEF), thus effectively decreasing the time, cost and handling error of the DNA extraction stage. The effectiveness of PEF treatment depended on the electrical conditions such as electric field strengths, total number of pulses (based on treatment time, pulse frequency). A maximum isolation was achieved when materials suspended in buffer (pH 5.5) was treated for 2 μs pulse width at 30 kV/cm, 140 Hz and n = 8, flow velocity = 2 ml/min, diluted time = 4×, 65 °C heating treatment in bipolar mode. Finally, PEF processing was two times more effective than untreated in extraction. This work clearly demonstrated that the PEF treatment at high-salted, low-acidic conditions was an effective method to achieve a high throughput DNA extraction.