Chiral separation of l,d-tyrosine and l,d-tryptophan by ct DNA

As one kind of chiral selector, calf thymus DNA (ct DNA) was used for the chiral separation of l,d-tyrosine and l,d-tryptophan. After mixed with ct DNA and dialyzed in water, l,d-tyrosine or l,d-tryptophan exhibited an obvious concentration difference in permeate solution. Their d-enantiomers preferentially existed in the permeate solution while l-enantiomers preferentially existed in the feed solution. This indicates that ct DNA prefers binding to l-enantiomers rather than binding to d-enantiomers in feed solution, and the binding between them is enantioselective.