Catalytic ethanolysis of soybean oil with immobilized lipase from Candida antarctica and 1H NMR and GC quantification of the ethyl esters (biodiesel) produced

▶ Catalytic ethanolysis of soybean oil by lipase from Candida antarctica. ▶ The parameters investigated were the quantity of biocatalyst, the reaction time, the amount of water added and turnover of lipase. ▶ The highest yields of biodiesel were obtained (87% by 1H NMR; 82.9% by GC). ▶ The quantification methods were efficient for determination of yields of ethyl esters (biodiesel) in mixtures with MAG, DAG, TAG.