Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6551083 | Forensic Science International | 2018 | 13 Pages |
Abstract
Urine samples are commonly analysed in cases with suspected illicit drug consumption. In events of alleged sample mishandling, urine sample source identification may be necessary. A simple DNA extraction procedure suitable for STR typing of urine samples was established on the Promega Maxwell® 16 paramagnetic silica bead platform. A small sample volume of 1.7 mL was used. Samples were stored at room temperature, 4 °C and â20 °C for 100 days to investigate the influence of storage temperature and time on extracted DNA quantity and success rate of STR typing. Samples stored at room temperature exhibited a faster decline in DNA yield with time and lower typing success rates as compared to those at 4 °C and â20 °C. This trend can likely be attributed to DNA degradation. In conclusion, this study presents a quick and effective DNA extraction protocol from a small urine volume stored for up to 100 days at 4 °C and â20 °C.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Huey Hian Ng, Hwee Chen Ang, See Ying Hoe, Mae-Lynn Lim, Hua Eng Tai, Richard Choon Hock Soh, Christopher Kiu-Choong Syn,