Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6551100 | Forensic Science International | 2018 | 21 Pages |
Abstract
Fingermarks can be exploited for both their ridge detail and touch DNA. One-step luminescent cyanoacrylate (CA) fuming techniques used for fingermark enhancement, such as PolyCyano UV (Foster + Freeman Ltd) and Lumicyano⢠(Crime Science Technology), claim to be compatible with DNA analysis as they reduce the need for postâstaining to increase contrast of the developed fingermark. The aim of this study was to determine the impact that these one-step luminescent cyanoacrylates have on DNA analysis and how they compare to conventional CA techniques. Four donors each deposited five sets of natural fingermarks, to which a known amount of washed saliva cells was dispensed onto half of each set of fingermarks. Each set was treated with either a conventional CA technique or a oneâstep luminescent CA technique prior to collection and processing of DNA, with one set left as a non-fumed control. It was found that DNA was still recoverable and detectable following each of the treatments. Lumicyano⢠had a similar impact on DNA profiles as conventional CA fuming and with postâstain, however, the degradation effect of PolyCyano UV on DNA was greater than the conventional treatments. For quantities of DNA such as that from touch DNA, the use of PolyCyano UV to enhance fingermarks may impact subsequent DNA analysis by causing allele drop out at larger fragment sizes.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Alicia Khuu, Scott Chadwick, Sébastien Moret, Xanthe Spindler, Peter Gunn, Claude Roux,